Computational Investigation of Growth Hormone Receptor Trp169Arg Heterozygous Mutation in a Child With Short Stature

作者：Porto William Farias; Marques Felipe Albuquerque; Pogue Huri Brito; de Oliveira Cardoso Maria Teresinha; Rodrigues do Vale Maria Gabriela; Pires Allan da Silva; Franco Octavio Luiz; de Alencar Sergio Amorim^*; Pogue Robert^*

来源：Journal of Cellular Biochemistry, 2017, 118(12): 4762-4771.

DOI：10.1002/jcb.26144

• GHR
• SHORT STATURE
• IN SILICO ANALYSIS
• DOCKING
• MOLECULAR DYNAMICS

Mutations in the growth hormone receptor (GHR) gene can cause disruption of the growth hormone signaling pathway, resulting in growth deficiency due to growth hormone (GH) resistance. Both recessive and apparently dominant mutations have been described in the literature. In order to shed some light on the molecular mechanism of partial growth hormone resistance caused by heterozygous mutations, we performed an in-depth in silico analysis of a mutation found in a girl with a previous diagnosis of idiopathic short stature. An array of algorithms was used to predict pathogenicity and potential impact on the protein, and molecular modeling, docking and dynamics were used to determine structural consequences. The results suggest that both of the possible single mutation-containing heteromeric GH-GHR complexes, as well as the double GHR mutant complex result in perturbation of complex structures, with altered ability of the GHR dimers to interact with the GH peptide. J. Cell. Biochem. 118: 4762-4771, 2017.

• 出版日期2017-12