A proposal for validation of antibodies

作者：Uhlen, Mathias^*; Bandrowski, Anita; Carr, Steven; Edwards, Aled; Ellenberg, Jan; Lundberg, Emma; Rimm, David L.; Rodriguez, Henry; Hiltke, Tara; Snyder, Michael; Yamamoto, Tadashi

来源：Nature Methods, 2016, 13(10): 823-+.

DOI：10.1038/NMETH.3995

• ASSAYS
• LOCALIZATION
• MICROARRAYS
• PROTEINS
• CELLS

We convened an ad hoc International Working Group for Antibody Validation in order to formulate the best approaches for validating antibodies used in common research applications and to provide guidelines that ensure antibody reproducibility. We recommend five conceptual 'pillars' for antibody validation to be used in an application-specific manner.

• 出版日期2016-10