A Fluorescence Resonance Energy Transfer Assay For Monitoring alpha-Synclein Aggregation in a Caenorhabditis Elegans Model For Parkinson's Disease

作者：Nagarajan Archana^*; Bodhicharla Rakesh; Winter Jody; Anbalagan Charumathi; Morgan Kevin; Searle Mark; Nazir Aamir; Adenle Ademola; Fineberg April; Brady Declan; Vere Kelly; Richens Jo; O'Shea Paul; Bell David; de Pomerai David

来源：CNS & Neurological Disorders-Drug Targets, 2015, 14(8): 1054-1068.

DOI：10.2174/1871527314666150821110538

• FRET
• C. elegans
• alpha-synuclein
• aggregation
• pollutants
• fluorescent proteins

The aggregation of alpha-synuclein (Syn or S) to form insoluble fibrils is important in the pathogenesis of Parkinson's disease, but key risk factors remain ill-defined. We have developed Fluorescence Resonance Energy Transfer (FRET)-based assays for alpha-synuclein aggregation, using Green Fluorescent Protein variants Cerulean (C) or Venus (V), fused to each other (CV, VC) or to human synuclein (SC, SV etc). Bacterially expressed proteins were purified to homogeneity, and C-terminal fusions SC and SV largely retained their ability to aggregate in vitro. FRET signals from mixtures of SC and SV were used to monitor aggregation. These fusion genes were linked to the C. elegans unc-54 myosin promoter to generate integrated transgenic strains. Increased FRET signals, indicative of S aggregation, were observed following treatment of unc-54::SC + unc-54::SV double transgenic worms with low concentrations of mercury or chlorpyrifos, or with RNAi against hsp-70 and hip-1. Opposite changes in Yellow Fluorescent Protein (YFP) fluorescence in an unc-54::SV strain (NL5901) are likely to reflect FRET from Yellow Fluorescent Protein to aggregates of Syn fusion protein. This could provide the basis for a high throughput screening assay, which could be used for studying the effects of toxic chemicals and environmental pollutants on the aggregation of proteins such as Syn in vivo.

• 出版日期2015