Differential Effects of Cocaine on Extracellular Signal-Regulated Kinase Phosphorylation in Nuclei of the Extended Amygdala and Prefrontal Cortex of Psychogenetically Selected Roman High- and Low-Avoidance Rats

作者：Giorgi Osvaldo; Corda Maria G; Sabariego Marta; Nano Valentina Ging; Piludu Maria A; Rosas Michela; Acquas Elio^*

来源：Journal of Neuroscience Research, 2015, 93(5): 714-721.

DOI：10.1002/jnr.23526

• extracellular signal-regulated kinase (ERK)
• nucleus accumbens
• prefrontal cortex
• psychogenetic selection
• Roman high- and low-avoidance rats

Roman high (RHA)-and low (RLA)-avoidance rats are selectively bred for rapid vs. poor acquisition of active avoidance, respectively, and differ markedly in emotional reactivity, coping style, and behavioral and neurochemical responses to morphine and psychostimulants. Accordingly, acute cocaine induces more robust increments in locomotion and dopamine output in the nucleus accumbens shell (AcbSh) of RHA than of RLA rats. Cocaine induces short-and long-term neuronal plasticity via activation of the extracellular signal-regulated kinase (ERK) pathway. This study compares the effects of acute cocaine on ERK phosphorylation (pERK) in limbic brain areas of Roman rats. In RHA but not RLA rats, cocaine (5 mg/kg) increased pERK in the infralimbic prefrontal cortex and AcbSh, two areas involved in its acute effects, but did not modify pERK in the prelimbic prefrontal cortex and Acb core, which mediate the chronic effects of cocaine. Moreover, cocaine failed to affect pERK immunolabeling in the bed nucleus of stria terminalis pars lateralis and central amygdala of either line but increased it in the basolateral amygdala of RLA rats. These results extend to pERK expression previous findings on the greater sensitivity to acute cocaine of RHA vs. RLA rats and confirm the notion that genetic factors influence the differential responses of the Roman lines to addictive drugs. Moreover, they support the view that the Roman lines are a useful tool to investigate the molecular underpinnings of individual vulnerability to drug addiction.

• 出版日期2015-5