Accurate gene synthesis with tag-directed retrieval of sequence-verified DNA molecules

作者：Schwartz Jerrod J^*; Lee Choli; Shendure Jay

来源：Nature Methods, 2012, 9(9): 913-+.

DOI：10.1038/nmeth.2137

We present dial-out PCR, a highly parallel method for retrieving accurate DNA molecules for gene synthesis. A complex library of DNA molecules is modified with unique flanking tags before massively parallel sequencing. Tag-directed primers then enable the retrieval of molecules with desired sequences by PCR. Dial-out PCR enables multiplex in vitro clone screening and is a compelling alternative to in vivo cloning and Sanger sequencing for accurate gene synthesis.

• 出版日期2012-9