The Dominant Sequence Types of Vancomycin-Resistant Enterococcus faecium Among Transplantation Ward Patients

作者：Mlynarczyk A; Szymanek Majchrzak K; Kosykowska E; Grzybowska W; Tyski S; Mrowka A; Baczkowska T; Durlik M; Pacholczyk M; Chmura A; Ciszek M; Paczek L; Mlynarczyk G^*

来源：Transplantation Proceedings, 2011, 43(8): 3132-3134.

DOI：10.1016/j.transproceed.2011.08.005

Objectives. Vancomycin-resistant enterococci (VRE) frequently cause therapeutic problems and provide information about the epidemiological condition of the ward.
Materials and Methods. VRE isolated from patients on transplantation wards in 2007-2008 were compared using 2 molecular methods: RFLP-PFGE (restriction fragment length polymorphism-pulse field gel electrophoresis) and MLST (multilocus sequence typing).
Results. The analysis covered 29 Enterococcus faecium strains resistant to glycopeptides, each from a different patient. All organisms were typed using 2 molecular methods. MLST results were compared with an international base. The 30 examined strains belonged to 8 different worldwide known sequence types. All could be recognized as representatives of a single clonal complex CC17.
Conclusion. Both methods of typing appeared to be useful to asses the epidemiological condition of the investigated wards.

• 出版日期2011-10