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摘要
Carbohydrate microarray has become a powerful tool to explore the structure-function relationship between protein-carbohydrate, but it depends on enough reducing terminal oligosaccharides with aldehyde group. Nostoc oligosaccharides are a kind of oligo(1 -> 2)-alpha-D-glucopyranosyl-(1 -> 2)-beta-D-fructofuranoside. To acquire a full series of a-1,2-gluco-oligosaccharides from oligo(1 -> 2)-alpha-D-glucopyranosyl-(1-2)-beta-D-fructofuranoside, the terminal fructose of four Nostoc oligosaccharides were removed using acid hydrolysis methods under the condition of 0. 5 mol/L trifluoroacetic acid(TFA) treated at 95 degrees C for 9 min, respectively. Following hydrolyzed products were separated and purified using high performance liquid chromatography (HPLC), and then electrospray ionization-collision-induce dissociation tandem mass spectrometry (ESI-CIDMS/MS) and matrix assisted laser desorption ionization (MALDI) -MS were used to identify the removing of terminal fructose and sequenced, alpha-1,2-G1c5, G1c7, G1c8 and G1c9 were acquired. The mixture of NostocOcta and Nostoc-Hexa treated with 0. 5 mol/L TFA at 95 degrees C for 45 min were used to acquire low degree of polymerization(DP) of alpha-1, 2-gluco-oligosaccharides, which were separated with P2 column, ESI-MS and MALDI-MS were used to analysis these oligosaccharides, alpha-1, 2-gluco-oligosaccharides with the degree of polymerization 2, 3, 4 and 6 were obtained. Finally, full series of alpha-1-2-gluco-oligosaccharides from 2 mer to 9 mer were acquired successfully.
- 出版日期2014-12-10
- 单位江南大学
