科研之友
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摘要
Super paramagnetic SiO2 @ Fe3O4 microsphere with core-shell structure was prepared by the method of hydro-thermal and Stober method, and modified by (3-aminopropyl) triethoxysilane (APTES). The obtained amino-Fe3O4 @ SiO2 microsphere with controlled surface charge properties was characterized by SEM and TEM, and the surface electric property of amino-microsphere was investigated by Zeta potential measurement. The microsphere was used to extract DNA from human blood, and the solid phase extraction method by microspheres was developed. The mechanism of action between Aminated-Fe3O4 @ SiO2 microsphere and DNA was explored, and the gel electrophoresis and PCR test were done on the extraction product. The result showed that genomic DNA with high purity was successfully extracted from whole blood by using amino-Fe3O4 @ SiO2 microsphere, with the extraction rate about 70%, the saturated adsorption capacity for DNA was about 40 ng/mu g, and the elution could be directly used for further bio-analysis.
- 出版日期2014-11
- 单位厦门大学
