Differences in the Synthesis and Elimination of Phosphatidylethanol 16:0/18:1 and 16:0/18:2 After Acute Doses of Alcohol

作者：Hill Kapturczak Nathalie; Dougherty Donald M; Roache John D; Karns Wright Tara E; Javors Martin A

来源：Alcoholism: Clinical and Experimental Research , 2018, 42(5): 851-860.

DOI：10.1111/acer.13620

• PEth Homologues
• Pharmacokinetics
• Blood
• Ethanol

BackgroundThe purpose of this study was to examine the synthesis and elimination of phosphatidylethanol (PEth) 16:0/18:1 and 16:0/18:2 following the consumption of alcohol among 56 light and heavy drinkers.
MethodsA transdermal alcohol monitor was used to promote alcohol absence 7days prior, and 14days after, alcohol consumption in the laboratory. Participants consumed a 0.4 or 0.8g/kg dose of alcohol in 15minutes. Blood and breath samples were collected before, at various times up to 360minutes postconsumption, and 2, 4, 7, 11, and 14days after alcohol consumption. Initial rates of PEth synthesis, 360minutes area under the PEth pharmacokinetic curves (AUCs), and elimination half-lives were determined.
Results(i) Nonzero PEth levels were observed before alcohol dosing for most participants, despite 7days of alcohol use monitoring; (ii) 0.4 and 0.8g/kg doses of alcohol produced proportional increases in PEth levels in all but 1 participant; (iii) the initial rate of synthesis of both PEth homologues did not differ between the 2 doses, but was greater for PEth 16:0/18:2 than PEth 16:0/18:1 at both doses; (iv) the mean AUC of both PEth homologues was higher at 0.8g/kg than at 0.4g/kg; (v) the mean AUC of 16:0/18:2 was greater than that of PEth 16:0/18:1 at both alcohol doses; (vi) the mean half-life of PEth 16:0/18:1 was longer than that of PEth 16:0/18:2 (7.83.3 [SD] days and 6.4 +/- 5.0 [SD] days, respectively); and (vii) there were no sex differences in PEth 16:0/18:1 or 16:0/18:2 pharmacokinetics.
ConclusionsThe results of this study support the use of PEth 16:0/18:1 and 16:0/18:2 as biomarkers for alcohol consumption. Because of consistent pharmacokinetic differences, the levels of these 2 PEth homologues may provide more information regarding the quantity and recentness of alcohol consumption than either alone.

• 出版日期2018-5