Dark field nanoparticle tracking analysis for size characterization of plasmonic and non-plasmonic particles

作者：Wagner Thorsten^*; Lipinski Hans Gerd; Wiemann Martin

来源：Journal of Nanoparticle Research, 2014, 16(5): 2419.

DOI：10.1007/s11051-014-2419-x

• Nanoparticle tracking analysis
• Dark field microscopy
• Instrumentation development
• Surface plasmon resonance
• Particle characterization
• ImageJ

Dark field microscopy is a widely unknown method to measure the particle size distribution of diffusing nanoparticles by particle tracking. Here we demonstrate that by using the surface plasmonic resonance of Au nanoparticles, size differences of ca. 20 nm can be identified within the particle size distribution. For that purpose, we developed a software tool which helps to analyze color videos of diffusing nanoparticles retrieved from CCD or CMOS cameras. Polystyrene beads with a diameter of 100 and 200 nm were used to compare the results to those obtained with a well-established laser-based particle tracking system. The methodology will be discussed in the light of recent developments in the emerging field of optical nanoparticle tracking.

• 出版日期2014-5-1