Antinociceptive Activity of Atranorin in Mice Orofacial Nociception Tests

作者：Siqueira Rosana S; Bonjardim Leonardo R; Araujo Adriano A S; Araujo Bruno E S; Melo Marcelia G D; Oliveira Marilia G B; Gelain Daniel P; Silva Francilene A; DeSantana Josimari M; Albuquerque Junior Ricardo L C; Rocha Ricardo F; Moreira Jose C F; Antoniolli Angelo R; Quintans Junior Lucindo J

来源：Zeitschrift fur Naturforschung Section C-A Journal of Biosciences, 2010, 65(9-10): 551-561.

• Antioxidant
• Atranorin
• Nociception
• Orofacial Pain

Physicochemical characterization and antinociceptive and anti-inflammatory activities of atranorin (AT) extracted from Cladina kalbii Ahti in formalin- and capsaicin-induced orofacial pain and anti-inflammatory tests in rodents were studied. Physicochemical characterization showed that AT has the general formula C(19)H(18)O(8). Male Swiss mice were pretreated with AT (100, 200, and 400 mg/kg, i.p.), morphine (3 mg/kg, i.p.), or vehicle (0.9% saline with two drops of 0.2% Tween 80) before formalin (20 mu l. 2%) or capsaicin (20 mu l, 2.5 mu g) were injected into the right vibrissa. Our results showed that i.p. treatment with AT displayed marked inhibitory effects in different orofacial pain tests in mice. AT (400 mg/kg, i.p.) was effective in reducing the nociceptive face-rubbing behavioural response in both phases of the formalin test, which was also naloxone-sensitive. Additionally, AT produced a significant antinociceptive effect at all doses in the capsaicin test. Such results were unlikely to be provoked by motor abnormality, since AT-treated mice exhibited no performance alteration on the rota rod apparatus. AT exhibited significant anti-inflammatory activity in the acute model of inflammation (leukocyte migration to the peritoneal cavity), carrageenan- and arachidonic acid-induced hind paw edema in rats. Additionally, AT exhibited a dose-dependent antioxidant activity in vitro, as assessed by total radical-trapping antioxidant parameter and total antioxidant reactivity assays. All these findings suggest that AT might represent an important tool for the management of orofacial pain and/or inflammatory disorders.

• 出版日期2010-10