A non-coding function of TYRP1 mRNA promotes melanoma growth

作者：Gilot David^*; Migault Melodie; Bachelot Laura; Journe Fabrice; Rogiers Aljosja; Donnou Fournet Emmanuelle; Mogha Ariane; Mouchet Nicolas; Pinel Marie Marie Laure; Mari Bernard; Montier Tristan; Corre Sebastien; Gautron Arthur; Rambow Florian; El Hajj Petra; Ben Jouira Rania; Tartare Deckert Sophie; Marine Jean Christophe; Felden Brice; Ghanem Ghanem; Galibert Marie Dominique^*

来源：Nature Cell Biology, 2017, 19(11): 1348-+.

DOI：10.1038/ncb3623

Competition among RNAs to bind miRNA is proposed to influence biological systems. However, the role of this competition in disease onset is unclear. Here, we report that TYRP1 mRNA, in addition to encoding tyrosinase-related protein 1 ( TYRP1), indirectly promotes cell proliferation by sequestering miR-16 on non-canonical miRNA response elements. Consequently, the sequestered miR-16 is no longer able to repress its mRNA targets, such as RAB17, which is involved in melanoma cell proliferation and tumour growth. Restoration of miR-16 tumour-suppressor function can be achieved in vitro by silencing TYRP1 or increasing miR-16 expression. Importantly, TYRP1-dependent miR-16 sequestration can also be overcome in vivo by using small oligonucleotides that mask miR-16-binding sites on TYRP1 mRNA. Together, our findings assign a pathogenic non-coding function to TYRP1 mRNA and highlight miRNA displacement as a promising targeted therapeutic approach for melanoma.

• 出版日期2017-11