Antioxidant Supplementation Alters Cytokine Production From Monocytes

作者：Toumpanakis Dimitrios; Karatza Maria Helena; Katsaounou Paraskevi; Roussos Charis; Zakynthinos Spyros; Papapetropoulos Andreas; Vassilakopoulos Theodoros^*

来源：Journal of Interferon and Cytokine Research, 2009, 29(11): 741-748.

DOI：10.1089/jir.2008.0114

We studied in 10 healthy subjects the effect of chronic enteral supplementation of antioxidants (vitamins E, C, A, allopurinol, and N-acetylcysteine) on cytokine production by monocytes at rest, end exercise (60-min cycling at 60% of maximum oxygen consumption), and 60 min post-exercise (recovery). The percentage and the mean fluorescent intensity (MFI) of both unstimulated and lipopolysaccharide (LPS)-stimulated tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, and IL-6-producing monocytes were detected using flow cytometry. Antioxidants decreased the percentage of unstimulated IL-6-producing monocytes following exercise, while their MFI increased at rest. The percentage of LPS-stimulated monocytes increased after exercise and they produced more IL-6 both at rest and following exercise. The percentage of unstimulated and LPS-stimulated IL-1 beta-producing monocytes was not affected by antioxidants. The MFI of IL-1 beta-produced unstimulated monocytes was increased after antioxidants both at rest and following exercise. After antioxidants, LPS-stimulated monocytes produced more IL-1 beta following exercise. Antioxidants decreased the percentage of TNF-alpha spontaneously-produced monocytes following exercise, which produced more TNF-alpha at recovery. Antioxidants did not affect the percentage of LPS-stimulated monocytes producing TNF-alpha, while LPS-stimulated production of TNF-alpha increased both at rest and following exercise. Antioxidants differentially affect TNF-alpha, IL-1 beta, and IL-6 production by monocytes, with a general tendency of augmenting cytokine production.

• 出版日期2009-11