ANTI-OXIDATIVE, alpha-GLUCOSIDASE AND alpha-AMYLASE INHIBITORY ACTIVITY OF VITEX DONIANA: POSSIBLE EXPLOITATION IN THE MANAGEMENT OF TYPE 2 DIABETES

作者：Ibrahim Mohammed Auwal; Koorb****ly Neil Anthony; Islam Md Shahidul^*

来源：Acta Poloniae Pharmaceutica, 2016, 73(5): 1235-1247.

• anti-oxidative activity
• alpha-glucosidase
• alpha-amylase
• Vitex doniana
• type 2 diabetes

Vitex doniana is an important African medicinal plant traditionally used for the treatment of many diseases including type 2 diabetes (T2D). In this study, ethyl acetate, ethanol and aqueous extracts of the stem bark, root and leaf of V. doniana were analyzed for in vitro anti-oxidative activity and the results indicated that the ethanolic extract of the leaves had the best anti-oxidative activity. Subsequently, the ethanolic extract of the leaves was partitioned between hexane, dichloromethane, ethyl acetate and water. The aqueous fraction had a significantly (p < 0.05) higher phenolics content and also showed the best anti-oxidative activity within the fractions. Furthermore, the aqueous fraction demonstrated significantly (p < 0.05) more potent inhibitory activities against a-glucosidase and alpha-amylase than other fractions. Steady state kinetics analysis revealed that the aqueous fraction inhibited both alpha-glucosidase and alpha-amylase activities in a non-competitive manner with inhibition binding constant (Ki) values of 5.93 and 167.44 mu g/mL, respectively. Analysis of the aqueous fraction by GC-MS showed the presence of resorcinol, 4-hydroxybenzoic acid, 3,4,5-trimethoxyphenol and 2,4'-dihydroxychalcone identified by their mass fragmentation patterns and comparison to standard spectra. The results obtained in this study showed that V. doniana leaves have a good in vitro anti-T2D potential possibly elicited through phenolics.

• 出版日期2016-10