Delay of the egg activation process in the Black Tiger Shrimp Penaeus monodon by manipulation of magnesium levels in spawning water

作者：Pongtippatee Pattira; Luppanakane Roengsak; Thaweethamsewee Pinij; Kirirat Pornpimol; Weerachatyanukul Wattana; Withyachumnarnkul Boonsirm^*

来源：Aquaculture Research, 2010, 41(2): 227-232.

DOI：10.1111/j.1365-2109.2009.02322.x

• Penaeus monodon
• magnesium effects
• egg activation
• cortical rod
• polyploidy

The aim of this study was to determine whether magnesium (Mg(2+)) in seawater is required for egg activation of the black tiger shrimp Penaeus monodon and whether manipulation of Mg(2+) levels can be used to delay the process and thereby synchronize egg activation. Female P. monodon brood-stock were allowed to spawn in artificial seawater containing Mg(2+) at varying levels with respect to the normal (100%) level: 100%, 50%, 20% and 0%. Egg activation occurred normally at 100% Mg(2+), incompletely at 50% and 20% Mg(2+) levels and did not occur at all with 0% Mg(2+). The fertilization rate with 100% Mg(2+) was observed to be 83%, but fertilization failed to take place in all the other groups. The fertilization rate was restored from 0% to 76% following the 20% Mg(2+) level treatment when Mg(2+) levels returned to normal (100%) as soon as spawning was completed. This study suggests that the level of Mg(2+) in seawater plays a vital role in P. monodon egg activation, and that commencement of this process could be delayed by manipulation of the Mg(2+) level during and immediately after spawning.

• 出版日期2010-1-18