A new fluorescent based screening system for high throughput screening of drugs targeting HBV-core and HBsAg interaction

作者：Suresh V; Krishnakumar K A; Asha V V^*

来源：Biomedicine & Pharmacotherapy, 2015, 70: 305-316.

DOI：10.1016/j.biopha.2015.02.002

• Cell based assay system
• Fluorescence
• eGFP
• mRFP
• HBsAg
• HBV-core
• Lamivudine

The existing screening systems for anti-hepatitis B virus (anti-HBV) drug discovery is time-consuming mainly due to the laborious detection system it is using. A new fluorescence based screening system for high throughput anti-HBV drug discovery was created by tagging hepatitis B surface antigen (HBsAg) with monomeric red fluorescent protein and hepatitis B virus (HBV) core protein with enhanced green fluorescent protein. The two constructs were co-transfected on to Hep3B cells and the transfection was stabilized by fluorescent activated cell sorter (FACS). The fusion proteins expressed through the secretory protein pathway as evidenced by localization with ER-Tracker and tubulin tracker. The new system has given analogues results like that of conventional enzyme-linked immunosorbent assay (ELISA). Hence it can be of very high potential for large scale drug screening systems.

• 出版日期2015-3