Discovery of DNA Repair Inhibitors by Combinatorial Library Profiling

作者：Moeller Benjamin J; Sidman Richard L; Pasqualini Renata^*; Arap Wadih

来源：Cancer Research, 2011, 71(5): 1816-1824.

DOI：10.1158/0008-5472.CAN-10-2361

Small molecule inhibitors of DNA repair are emerging as potent and selective anticancer therapies, but the sheer magnitude of the protein networks involved in DNA repair processes poses obstacles to discovery of effective candidate drugs. To address this challenge, we used a subtractive combinatorial selection approach to identify a panel of peptide ligands that bind DNA repair complexes. Supporting the concept that these ligands have therapeutic potential, we show that one selected peptide specifically binds and noncompetitively inactivates DNA-PKcs, a protein kinase critical in double-strand DNA break repair. In doing so, this ligand sensitizes BRCA-deficient tumor cells to genotoxic therapy. Our findings establish a platform for large-scale parallel screening for ligand-directed DNA repair inhibitors, with immediate applicability to cancer therapy. Cancer Res; 71(5); 1816-24.

• 出版日期2011-3-1