Anti-inflammatory effect of Qingwen Baidu Decoction in sepsis rats

作者：Yu Zheng-miao^*; Liu Zhi-hui; Chen Jing; Zeng Qiang

来源：Chinese Journal of Integrative Medicine, 2014, 20(12): 934-943.

DOI：10.1007/s11655-014-1863-x

• sepsis
• summer heat syndrome
• Qingwen Baidu Decoction
• cytokine
• mediator network
• Chinese medicine

To explore the pharmacological anti-inflammatory mechanism of Chinese formula Qingwen Baidu Decoction (ae...c similar to Ye ' yenae-'eyen (R), QBD) from the view of holistic biology. @@@ The rats were randomly divided into a normal conrol group, a lipopolysaccharide (LPS) group, the low- and high-dose QBD groups, and a dexamethasone (DXM) group. NR8383 cells were treated with culture fluid containing 6% serum from rats of each group respectively. Inflammatory mediators were detected by reverse transcription polymerase chain reaction (RT-PCR), Western blotting hybridization, enzyme linked immunosorbent assay (ELISA), polymerase chain reaction (PCR) gene array and antibody array. @@@ It is showed that the levels of interleukin (IL)-1 alpha, IL-4 and IL-12 were enhanced in the low-dose QBD group; levels of IL-1 alpha, IL-12 and IL-18 were augmented in the high-dose QBD group, compared with the LPS group after ELISA detection. Western blot showed that IL-1 beta and tumor necrosis factor (TNF)-alpha expression of the control group were lower than other groups. IL-1 beta level of the low-dose and high-dose QBD groups detected by RT-PCR was higher in early stage but lower after 24 h than that of the control group (P < 0.01). Expression of 84 main inflammatory cytokines and receptors was detected by rat inflammatory cytokines and receptors PCR array. Up-regulation genes were 22 in both the LPS group and the low-dose QBD group, among which 16 up-regulating genes were the same. In these 16 genes, the up-regulating amplitude of 9 genes in the low-dose QBD group was less than that in the LPS group, 4 were similar to and 3 were more. Twenty-nine main cytokines were inspected by rat cytokine antibody array. Intergroup gray value differences were found in 7 expressed cytokines. The levels of these 7 cytokines in the lowdose QBD group were all lower than those in the the LPS group. @@@ QBD has anti-inflammatory effect on sepsis by changing the level of inflammatory mediators.

• 出版日期2014-12
• 单位广州中医药大学; 广东工业大学; 广州中医药大学第一附属医院