A functional role for intrinsic disorder in the tau-tubulin complex

作者：Melo Ana M; Coraor Juliana; Alpha Cobb Garrett; Elbaum Garfinkle Shana; Nath Abhinav; Rhoades Elizabeth^*

来源：Proceedings of the National Academy of Sciences, 2016, 113(50): 14336-14341.

DOI：10.1073/pnas.1610137113

• single-molecule FRET
• intrinsically disordered proteins
• microtubule-associated protein
• tauopathies
• Alzheimer's disease

Tau is an intrinsically disordered protein with an important role in maintaining the dynamic instability of neuronal microtubules. Despite intensive study, a detailed understanding of the functional mechanism of tau is lacking. Here, we address this deficiency by using intramolecular single-molecule Forster Resonance Energy Transfer (smFRET) to characterize the conformational ensemble of tau bound to soluble tubulin heterodimers. Tau adopts an open conformation on binding tubulin, in which the long-range contacts between both termini and the microtubule binding region that characterize its compact solution structure are diminished. Moreover, the individual repeats within the microtubule binding region that directly interface with tubulin expand to accommodate tubulin binding, despite a lack of extension in the overall dimensions of this region. These results suggest that the disordered nature of tau provides the significant flexibility required to allow for local changes in conformation while preserving global features. The tubulin-associated conformational ensemble is distinct from its aggregation-prone one, highlighting differences between functional and dysfunctional states of tau. Using constraints derived from our measurements, we construct a model of tubulin-bound tau, which draws attention to the importance of the role of tau's conformational plasticity in function.

• 出版日期2016-12-13