alpha 4-integrin(+) endothelium derived from primate embryonic stem cells generates primitive and definitive hematopoietic cells

作者：Shinoda Gen; Umeda Katsutsugu; Heike Toshio; Arai Masato; Niwa Akira; Ma Feng; Suemori Hirofumi; Luo Hong Yuan; Chui David H K; Torii Ryuzo; Shibuya Masabumi; Nakatsuji Norio; Nakahata Tatsutoshi^*

来源：Blood, 2007, 109(6): 2406-2415.

DOI：10.1182/blood-2006-06-031039

The mechanism of commencement of hematopolesis in blood islands of the yolk sac and the aorta-gonad-mesonephros (AGM) region during primate embryogenesis remains elusive. In this study, we demonstrated that VE-cadherin(+)CD45(-)endothelial cells derived from nonhuman primate embryonic stem cells are able to generate primitive and definitive hematopoietic cells sequentially, as revealed by immunostaining of floating erythrocytes and colony-forming assay in cultures. Single bipotential progenitors for hematopoietic and endothelial lineages are included in this endothelial cell population. Furthermore, hemogenic activity of these endothelial cells is observed exclusively in the alpha 4-integrin(+) subpopulation; bipotential progenitors are 4-fold enriched in this subpopulation. The kinetics of this hemogenic subpopulation is similar to that of hemogenic endothelial cells previously reported in the yolk sac and the AGM region in vivo in that they emerge for only a limited time. We suggest that VE-cadherin(+)CD45(-)alpha 4-integrin(+) endothelial cells are involved in primitive and definitive hemtopoiesis during primate embryogenesis, though VE-cadherin(-)CD45(-)alpha 4-integrin(+) cells are the primary sources for primitive hematopolesis.

• 出版日期2007-3-15
• 单位河北医科大学