摘要
Drug-drug interaction is one of the Most important preclinical investigation directions, It may have serious impact on drug absorption, distribution and elimination through Membrane transporters, Liquid chromatography/Tandem Mass spectrometry (LC/MS/MS) Could be used to investigate drug drug interaction in vitro. A sensitive LC/MS/MS method was established for quantification of rosuvastatin in HEK293 and S2 cell lysates. Internal standard (IS) was indapamide. Detection mode was positive eleetrospray ionization. The lower limit of quantification (LLOQ) was 0.05 ng/mL. The affinity of rosuvastatin with organic anion transporting polypeptides 1B1 and 2B1 (OATP1B1 and OATP2B1) was investigated through uptake experiment, All cell samples were precipitated with methanol. Analytes were separated by reversed-phase chromatography and analyze by Tandem mass spectrometry using m/z 482.2/258.1 for rosuvastatin and m/z 366.1/132.1 for indapamide. Rosuvastatin uptake was saturable with KM value of 11.70 and 5.98 mu M for OATP1B1 and OATP2B1. Rosuvastatin could be used as a good probe for studying membrane transporter activity and drug-drug interaction through LC/MS/MS method.
- 出版日期2014-1
- 单位天津药物研究院有限公司; 天津大学