A novel snoRNA gene cluster in yeast is transcribed as polycistronic pre-snoRNAs

作者：Lu, YJ; Zhou, H; Zhou, WX; Zhu, YQ; Qu, LH^*

来源：Science China Life Sciences, 1999, 42(5): 529-537.

DOI：10.1007/BF02881777

• Z8 snoRNA
• 2 '-O-ribose methylation
• polycistron
• Saccharomyces cerevisiae

Small nucleolar RNAs (snoRNAs) play an important role in eukaryotic rRNA biogenesis. By combination of a computer search of EMBL database and experimental procedure, a novel snoRNA coding sequence (28) was screened out and characterized from yeast Saccharomyces cerevisiae genome. Z8 snoRNA gene codes a boxC/D antisense snoRNA which guides, deduced from structure analysis, the 2'-O-ribose methylation at U-2421 Of 25S rRNA. After disruption of 28 snoRNA gene, the methylation at corresponding site was abolished, but no growth delay was observed in various cultural temperatures. 28 DNA is the first gene of a gene cluster consisting of three cognate snoRNA genes which are located on an intergenic region of chromosome XIII. This gene cluster is co-transcribed as a polycistronic precursor from a 247 bp U snoRNA gene promoter, followed by processing to release individual snoRNAs, representing a new expression pattern of snoRNA genes.

• 出版日期1999-10
• 单位中山大学