Mechanism of Cu(A) assembly

作者：Abriata Luciano A; Banci Lucia; Bertini Ivano^*; Ciofi Baffoni Simone; Gkazonis Petros; Spyroulias Georgios A; Vila Alejandro J; Wang Shenlin

来源：Nature Chemical Biology, 2008, 4(10): 599-601.

DOI：10.1038/nchembio.110

Copper is essential for proper functioning of cytochrome c oxidases, and therefore for cellular respiration in eukaryotes and many bacteria. Here we show that a new periplasmic protein (PCu(A)C) selectively inserts Cu(I) ions into subunit II of Thermus thermophilus ba(3) oxidase to generate a native Cu(A) site. The purported metallochaperone Sco1 is unable to deliver copper ions; instead, it works as a thiol-disulfide reductase to maintain the correct oxidation state of the CuA cysteine ligands.

• 出版日期2008-10