Protection against renal disease in (NZB x NZW)F-1 lupus-prone mice after somatic B cell gene vaccination with anti-DNA immunoglobulin consensus peptide

作者：Ferrera Francesca; Hahn Bevra H; Rizzi Marta; Anderson Marissa; FitzGerald John; Millo Enrico; Indiveri Francesco; Shi Fu Dong; Filaci Gilberto; La Cava Antonio^*

来源：Arthritis and Rheumatism, 2007, 56(6): 1945-1953.

DOI：10.1002/art.22700

Objective. Ig molecules contain epitopes that can induce T cell-mediated immune responses. B cells can process and present such epitopes and activate T cells. The purpose of the present study was to test our hypothesis that T cells that recognize an Ig consensus sequence presented by B cells will modulate lupus-like disease in mice.
Methods. (NZB x NZW)F-1 (NZB/NZW) lupus mice received somatic B cell gene transfer of a DNA plasmid encoding a consensus sequence of T cell determinants of murine anti-DNA IgG or control plasmids. Treated animals were monitored for the production of antibody, the development of renal disease, and the phenotype, number, and function of T cells.
Results. Treatment of mice with Ig consensus plasmid induced transforming growth factor beta-producing CD8+,CD28- T cells that suppressed the antigen-specific stimulation of CD4+ T cells in a cell-contact-independent manner, reduced antibody production, retarded the development of nephritis, and improved survival. Significantly, adoptive transfer of CD8+,CD28- T cells from protected mice into hypergammaglobulinemic NZB/NZW mice effectively protected the transferred mice from the development of renal disease.
Conclusion. Gene expression of anti-DNA Ig consensus sequence induces immunoregulatory T cells that delay the development of lupus nephritis by suppressing hypergammaglobulinemia and renal disease.

• 出版日期2007-6