Analysis of a chaotic micromixer by novel methods of particle tracking and FRET

作者：Tung Kai Yang; Yang Jing Tang

来源：Microfluidics and Nanofluidics, 2008, 5(6): 749-759.

DOI：10.1007/s10404-008-0287-3

• Circulation-disturbance micromixer (CDM)
• Constructive interference
• Particle tracking
• Fluorescence resonance energy transfer (FRET)

Two novel numerical and experimental methods are proposed for quantitatively analyzing the chaotic mixing in a circulation-disturbance micromixer (CDM). A particle-tracking approach incorporated with the geometric distribution of particles is derived to evaluate the mixing performance. This method provides additional evaluation about the degree of particles scattering, when compared to the Shannon entropy method. In addition, the fluorescence resonance energy transfer (FRET) method using R-Phycoerythrin (RPE) and cross-linked allophycocyanin (clAPC) is developed to demonstrate both the mixing performance and the applicability of CDM for biochemical reaction. The two proposed indices, including the particle-tracking distribution and FRET factor, are verified to sufficiently reveal the periodic constructive interference of the vortices in a CDM and a slanted groove micromixer. They also explicitly indicate the mixing performance of the chaotic micromixers. The methodologies are expected to be applied successfully for performance analysis of various micromixers or microreactors, and thus have a great potential for mu TAS applications.

• 出版日期2008-12
• 单位清华大学