Novel and highly effective chemoenzymatic synthesis of (2R)-2-[4-(4-cyano-2-fluorophenoxy)phenoxy]butylpropanoate based on lipase mediated transesterification

作者：Zhu, Genlin; Yao, Risheng; Zhu, Huixia; Wang, Huai^*

来源：Biotechnology Letters, 2012, 34(4): 709-715.

DOI：10.1007/s10529-011-0820-4

• Candida antarctica lipase B
• Chemoenzymatic synthesis
• Cyhalofop-butyl
• Lipase
• Transesterification

(2R)-2-[4-(4-Cyano-2-fluorophenoxy)phenoxy]butylpropanoate (cyhalofop-butyl, CyB) was synthesized by a chemoenzymatic route involving enantioselective transesterification with Candida antarctica lipase B (Novozym 435). The optimum organic solvent, acyl donor, a (w) , reaction temperature and shaking rate for the transesterification were acetonitrile, n-butanol, 0.11, 45A degrees C and 200 rpm, respectively. Under the optimum conditions, the maximum substrate conversion and the enantiomeric purity of the product were 96.9 and > 99%, respectively. The total yield and enantiomeric purity of CyB by this chemoenzymatic synthesis were 60.4 and > 99%, respectively; 15.3 and 21% higher than that of the traditional way (45 and 78%).

• 出版日期2012-4
• 单位合肥工业大学