Proteomic comparison between interphase and metaphase of isolated chloroplasts of Cyanidioschyzon merolae (Cyanidiophyceae, Rhodophyta)

作者：Yoshida Masaki^*; Yoshida Yamato; Fujiwara Takayuki; Misumi Osami; Kuroiwa Haruko; Kuroiwa Tsuneyoshi

来源：Phycological Research, 2011, 59(1): 1-15.

DOI：10.1111/j.1440-1835.2010.00589.x

• chloroplast
• Cyanidioschyzon merolae
• matrix-assisted laser desorption
• ionization time-of-flight mass spectrometry
• organelle isolation
• proteome

P>With the completion of the Cyanidioschyzon merolae genome project, detailed analysis of organelle proteins with mass spectrometry has now become possible. Chloroplasts of the unicellular red alga Cyanidioschyzon merolae De Luca, Taddei et Varano (Rhodophyta, Cyanidiophyceae) were isolated from synchronized culture and the chloroplast lysates of both interphase and metaphase cells were prepared and subjected to 2D-polyacrylamide gel electrophoresis. A total of 355 spots (170 identical spots) were recognized and quantified and then analyzed using mass spectrometry. A total of 105 proteins were identified, including 18 proteins for posttranslational functions, 17 photosynthesis-related proteins, 10 carbohydrate-related proteins, 15 proteins of unknown functions, and eight proteins predicted to be contaminated from other organelles. On the basis of spot quantity, photosynthesis-related proteins were most dominant (45.3% in interphase and 56.4% in metaphase). In particular, the proteins forming phycobilisomal complexes were abundant. Comparison of interphase with metaphase revealed that CMG086C (aminomethyltransferase) notably increased in interphase. CMN235C (similar to chlorophyll a/b-binding protein, CP24) increased in metaphase in agreement with a previously performed microarray analysis. Both CMQ295C (cell division protein FtsH) and CMS004C (plastid division protein FtsZ) increased in interphase. Seven proteins were detected to be interphase-specific, and 12 proteins were metaphase-specific. Proteins of unknown functions were poorly characterized by homology search, although thioredoxin-like domains were predicted in several proteins.

• 出版日期2011-1