A New Strategy for Synthesis of "Umbrella-Like" Poly(ethylene glycol) with Monofunctional End Group for Bioconjugation

作者：Zhang Yannan; Wang Guowei; Huang Junlian^*

来源：Journal of Polymer Science Part A: Polymer Chemistry , 2010, 48(24): 5974-5981.

DOI：10.1002/pola.24417

• branched poly(ethylene glycol)
• protein conjugation
• ring opening polymerization
• star polymers
• umbrella like structure

A novel strategy was used to synthesize poly(ethyl ene glycol) (PEG) with umbrella like structure containing a single reactive group at the handle of the umbrella 1 (Bis(2 hydroxyethyl)amino) 3 (1 ethoxyethoxy)propan 2 ol was used to initiate the ring opening polymerization (ROP) of ethyl ene oxide (EO) in the presence of diphenylmethylpotassium (DPMK) to obtain three arm PEG (PEG3) then terminated by benzyl bromide or ethyl bromide The resultant PEG3 was hydrolyzed to generate hydroxyl group at the conjunction point and the second step ROP of EO was carried out using PEG3 OH as macroinitiator in the presence of DPMK The obtained four arm PEG (PEG4) contained a functional hydroxyl group at the end of the fourth arm which could be easily modified to bioactive groups such as carboxyl active ester amino etc The well defined structure of umbrella like PEG was characterized by GPC (1)H NMR and MALDI TOF MS i

• 出版日期2010-12-15
• 单位复旦大学