Direct measurement of the dielectrophoresis forces acting on micro-objects using optical tweezers and a simple microfluidic chip

作者：Park In Soo; Park Se Hee; Yoon Dae Sung; Lee Sang Woo^*; Kim Beop Min

来源：Applied Physics Letters, 2014, 105(10): 103701.

DOI：10.1063/1.4895115

We constructed a reliable frequency-dependent dielectrophoretic (DEP) force measurement system based on optical tweezers and a microfluidic chip. Using this system, we directly measured the frequency-dependent DEP forces acting on polystyrene beads while varying various parameters, which were all verified by theoretical simulations. We also investigated the DEP characteristics of non-functionalized and carboxyl-functionalized polystyrene beads in solutions with different conductivities by associating the measured crossover frequencies with a theoretical DEP model. This system can be used as a quantifying tool for surface conductance assays by characterizing the DEP forces acting on micro-objects in various experimental conditions.

• 出版日期2014-9-8