Antimicrobial Peptide Delivery from Trabecular Bone Grafts

作者：Kazemzadeh Narbat Mehdi; Wang Qiong; Hancock Robert E W; Wang Rizhi^*

来源：Journal of Biomaterials and Tissue Engineering, 2014, 4(11): 967-972.

DOI：10.1166/jbt.2014.1272

• Bone Scaffolds
• Allografts
• Antimicrobial Peptides
• Drug Delivery

Autografts and allografts are extensively used for reconstruction of skeletal defects caused by trauma, tumor resection, and other diseases. They are also often used together with total hip and total knee implants. Similar to synthetic implants, these bone grafts also face the risk of infection. In this report, we propose to combine bone autografting and allografting with local delivery of antimicrobial agents to combat pen-implant infections. A special group of antimicrobial peptides was selected for this purpose to address the concern of developing antibiotic resistance. Both natural and sintered trabecular bone specimens were impregnated with a short cationic antimicrobial peptide HHC-36 (KRWWKWWRR). Antimicrobial activity using a survival assay against Gram-positive bacterium, S. aureus confirmed that the natural bone scaffolds could kill 100% of bacterial cells, while the sintered scaffolds could reduce the number of bacterial cells by 99% within four hours of culture. Such a difference is attributed to the peptide loading efficiency in the natural bone scaffolds which is five times as high as that in the sintered trabecular bone specimens.

• 出版日期2014-11