Application of real-time quantification polymerase chain reaction in detecting the expression of BCR-ABL in chronic myelogenous leukemia cells

作者：WU Wei; HAN Ruiling; ZHANG Li; LI Yan

来源：检验医学与临床, 2016, 13(22).

DOI：10.3969/j.issn.1672-9455.2016.22.001

• chronic myelogenous leukemia
• BCR-ABL
• real-time quantification polymerase chain reaction

Objective To explore the clinical significance of real‐time quantitative polymerase chain reaction (RQ‐PCR) in detec‐ting BCR‐ABLp210 fusion gene in chronic myelogenous leukemia (CML ) cells .Methods The transcript level of BCR‐ABLp210 was measured by RQ‐PCR from the bone marrow cells of 60 CML patients .Results All of 60 CML patients were BCR‐ABL posi‐tive .Among the 60 patients ,33 patients were in chronic phase ,13 patients were in accelerated phase and 14 patients were in blast phase .In contrast ,the transcript level of BCR‐ABLp210 in blast phase was significantly higher than that in the other phases .Six months after the treatment with allogenic hematopoietic stem cell transplantation (allo‐HSCT ) or imatinib ,the transcript level of BCR‐ABLp210 was decreased .In addition ,there was no significant difference between allo‐HSCT and imatinib treatment after 12 months(P > 0 .05) .Meanwhile ,the application of the other tyrosine kinase inhibitors had the similar results for CML after 12‐month treatment .Conclusion The detection of BCR‐ABLp210 transcript level by RQ‐PCR contributes to the diagnosis ,the evalua‐tion of curative effect and the minimal residue detection .

• 出版日期2016
• 单位武汉大学