Achromobacter aloeverae sp nov., isolated from the root of Aloe vera (L.) Burm.f

作者：Kuncharoen Nattakorn; Muramatsu Yuki; Shibata Chiyo; Kamakura Yuki; Nakagawa Yasuyoshi; Tanasupawat Somboon^*

来源：International Journal of Systematic and Evolutionary Microbiology, 2017, 67(1): 37-41.

DOI：10.1099/ijsem.0.001566

• Alcaligenaceae
• beta-proteobacteria
• Achromobacter
• plant root

Two Gram-staining-negative, strictly aerobic, rod-shaped bacteria, designated strains AVA-1T and AVA-2, were isolated from the root of Aloe vera (L.) Brum.f. derived from Chachoengsao Province, Thailand. The strains contained cytochrome oxidase and catalase activities. They grew in 4% (w/v) NaCl, at a pH range of 6.0-9.0 (optimally at pH 7) and at 20-42 degrees C (optimally at 30-37 degrees C). The major isoprenoid quinone was ubiquinone with eight isoprene units (Q-8). The major fatty acids were C-16:0 and C-17:0 cyclo. On the basis of 16S rRNA gene sequence analysis, the strains represent a species belonging to the genus Achromobacter and are closely related to Achromobacter xylosoxidans NBRC 15126(T) (98.80%), Achromobacter insolitus LMG 6003(T) (98.64%), Achromobacter aminicus LMG 26690(T) (98.59%), Achromobacter pulmonis LMG 26696(T) (98.58%) and Achromobacter insuavis LMG 26845(T) (98.58%). The DNA G+C content of strain AVA-1(T) was 66.5 mol%. The novel strains had low DNA-DNA relatedness values with related type strains. On the basis of the phenotypic and genotypic data obtained, the strains clearly represent a novel species, for which the name Achromobacter aloeverae sp. nov. is proposed. The type strain is strain AVA-1(T) (=LMG 29108(T) =NBRC 111463(T) =PCU 352(T) =TISTR 2383(T)).

• 出版日期2017-1