A never defensin from the lentil Lens culinaris seeds

作者：Finkina Ekaterina I; Shramova Elena I; Tagaev Andrey A; Ovchinnikova Tatiana V^*

来源：Biochemical and Biophysical Research Communications, 2008, 371(4): 860-865.

DOI：10.1016/j.bbrc.2008.04.161

• defensin
• lentil
• Lens culinaris
• germinated seeds
• primary structure
• molecular cloning
• antifungal activity

A novel 47-residue plant defensin was purified from germinated seeds of the lentil Lens culinaris by ammonium sulfate precipitation, gel filtration, chromatography, and RP-HPLC. The molecular mass (5440.41 Da) and complete amino acid sequence (KTCENLSDSFKGPCIPDGNCNKHCKEKEHLLSGRCRDD FRCWCTRNC)(1) of defensin, termed Lc-def, were determined. Lc-def has eight cysteines forming four disulfide bonds. The total RNA was isolated from lentil germinated seeds, RT-PCR and subsequent cloning were performed, and cDNA was sequenced. A 74-residue predefensin contains a putative signal peptide (27 amino acid) and a mature protein. Lc-def shows high sequence homology with legumes defensins, exhibits an activity against Aspergillus niger, but does not inhibit proteolytic enzymes.

• 出版日期2008-7-11