Detecting changes in arabidopsis cell wall composition using time-of-flight secondary ion mass spectrometry

作者：Tsai Alex Yi Lin; Goacher Robyn E; Master Emma R^*

来源：Surface and Interface Analysis, 2015, 47(5): 626-631.

DOI：10.1002/sia.5756

• Time-of-flight secondary ion mass spectrometry (ToF-SIMS)
• arabidopsis thaliana
• lignocellulose
• cellulose
• hemicellulose
• lignin

Time-of-flight secondary ion mass spectrometry (ToF-SIMS) was previously used to characterize lignocellulosic materials, including woody biomass. ToF-SIMS can acquire both rapid spectral and spatial information about a sample's surface composition. In the present study, ToF-SIMS was used to characterize the cell walls of stem tissue from the plant model organism, Arabidopsis thaliana. Using principal component analyses, ToF-SIMS spectra from A. thaliana wild-type (Col-0), cellulose mutant (irx3), and lignin mutant (fah1) stem tissues were distinguished using ToF-SIMS peaks annotated for wood-derived lignocellulose, where spectra from the irx3 and fah1 were characterized by comparatively low polysaccharide and syringyl lignin content, respectively. Spatial analyses using ToF-SIMS imaging furthermore differentiated interfascicular fiber and xylem vessels based on differences in the lignin content of corresponding cell walls. These new data support the applicability of ToF-SIMS peak annotations based on woody biomass for herbaceous plants, including model plant systems like arabidopsis.

• 出版日期2015-5