Association analysis of thromboxane A synthase 1 gene polymorphisms with aspirin intolerance in asthmatic patients

作者：Oh Sun Hee; Kim Yong Hoon; Park Se Min; Cho Sung Hwan; Park Jong Sook; Jang An Soo; Park Sung Woo; Uh Soo Taek; Lee Youg Mok; Kim Mi Kyeong; Choi Inseon S; Cho Sang Heon; Hong Chein Soo; Lee Yong Won; Lee Jae Young; Choi Byoung Whui; Park Byung Lae; Shin Hyoung Doo; Park Choon Sik^*

来源：Pharmacogenomics, 2011, 12(3): 351-363.

DOI：10.2217/PGS.10.181

• aspirin
• asthma
• gene
• isoform
• SNP
• thromboxane A synthase 1

Aim: Thromboxane A synthase (TBXAS1) converts prostaglandin H to thromboxane A, a potent constrictor of smooth respiratory muscle. Thus, functional alterations of the TBXAS1 gene may contribute to aspirin-intolerant asthma (AIA). Materials & methods: We investigated the relationship between SNPs in the TBXAS1 gene and AIA. Asthmatics (n = 470) were categorized into AIA (20% or greater decreases in forced expiratory volume in 1 s [FEV1], or 15% to 19% decreases in FEV1 with naso-ocular or cutaneous reactions) and aspirin-tolerant asthma (ATA). A total of 101 SNPs were genotyped. mRNA expression of the TBXAS1 gene by peripheral blood mononuclear cells and plasma thromboxane B2 (TXB2) concentrations were measured by reverse transcriptase (RT)-PCR and ELISA. Results: Logistic regression analysis showed that the rare allele frequency of rs6962291 in intron 9 was significantly lower in the AIA group (n = 115) than in the ATA group (n = 270) (p(corr) = 0.04). The linear regression analysis revealed a strong association of rs6962291 with the aspirin challenge-induced FEV1 fall (p = 0.003). RT-PCR revealed an exon-12-deleted splice variant. We measured TBXAS1 mRNA levels in peripheral blood mononuclear cells. The mRNA levels of the full-length wild-type and splice variant were significantly higher in the TT homozygotes than in the AA homozygotes of rs6962291 (1.00 +/- 0.18 vs 0.57 +/- 0.03 and 1.00 +/- 0.18 vs 0.21 +/- 0.05, p = 0.047 and 0.001, respectively). The plasma TXB2 level was significantly lower in rs6962291 AA carriers than in rs6962291 IT (p = 0.016) carriers. Conclusion: The rare allele of rs6962291 may play a protective role against aspirin hypersensitivity via a lower catalytic activity of the TBXAS1 gene, attributed to the increase of a nonfunctioning isoform of TBXAS1.

• 出版日期2011-3
• 单位河北医科大学