科研之友
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摘要
Purpose: To investigate lacrimal gland (LG) immunophysiological and immune-mediated inflammatory process (IMIP) phenotype diversity. Methods: Ex vivo matured dendritic cells (mDC) were loaded with acinar cell microparticles (M-P). Peripheral blood lymphocytes (PBL) were activated in mixed cell reactions with mDC and injected directly into autologous, unilateral LG (1 degrees ATD-LG) of two rabbit cohorts, one naive, one immunized with a LG lysate membrane fraction (P-i). Autoimmune IgG titers were assayed by ELISA, MCR PBL stimulation indices (SI) by [H-3]-thymidine incorporation. Schirmer tests without and with topical anesthetic (STT-I, STT-I-A) and rose Bengal (RB) staining tests were performed. H&E and immunohistochemically stained sections were examined. RNA yields and selected transcript abundances were measured. Immune cell number and transcript abundance data were submitted to Principal Component Analysis (PCA). Results: Immunizing Pi dose influenced SI but not IgG titers. STT scores were decreased, and rose Bengal scores increased, by day 118 after immunization. Previous immunization exacerbated scores in 1 degrees ATD-eyes and exacerbated 1 degrees ATD-LG atrophy. IMIP were evident in 2 degrees ATD-LG as well as 1 degrees ATD-LG. PCA described diverse immunophysiological phenotypes in control LG and diverse IMIP phenotypes in ATD-LG. IgG titers and SI pre-adoptive transfer were significantly associated with certain post-adoptive transfer IMIP phenotype features, and certain LG IMIP features were significantly associated with RB and STT I-A scores. Conclusions: The underlying variability of normal states may contribute to the diversity of experimental IMIP phenotypes. The ability to generate and characterize diverse phenotypes may lead to phenotype-specific diagnostic and therapeutic paradigms.
- 出版日期2016-10
