Association of organochlorine pesticides with the mRNA expression of tumour necrosis factor-alpha (TNF-alpha) & cyclooxygenase-2 (COX-2) genes in idiopathic preterm birth

作者：Tyagi Vipin; Mustafa M D; Sharma Tusha; Banerjee B D^*; Ahmed Rafat S; Tripathi A K; Guleria Kiran

来源：Indian Journal of Medical Research, 2016, 143(6): 731-738.

DOI：10.4103/0971-5916.191986

• COX-2
• gene-environment interaction
• mRNA expression
• organochlorine pesticides
• preterm birth
• TNF-alpha

Background & objectives: Preterm birth (PTB) is an important cause of prenatal death, neonatal morbidity and mortality and adult illness. Increased inflammation occurs in normal parturition, and inflammatory cytokines and oxidative stress are found to be higher in PTB cases. The present study was planned to investigate the association of organochlorine pesticides (OCPs) with mRNA expression of inflammatory pathway genes such as tumour necrosis factor-alpha (TNF-alpha) and cyclooxygenase-2 (COX-2) in preterm delivery (PTD) cases.
Methods: Maternal blood samples of PTD (n=30) cases and equal number of term delivery (n=30) were collected at the time of labour. Women occupationally exposed to OCPs and other high risk factors such as anaemia, hypertension, bacterial vaginosis, renal and heart disease, diabetes, etc. were excluded. The OCP levels were estimated by gas chromatography, and mRNA expressions of TNF-alpha and COX-2 genes were analysed using real-time PCR (qPCR).
Results: Significantly higher levels of beta-HCH (beta-hexachlorocyclohexane, 95% CI=2.08-4.633, P=0.001), p'p'-DDE (para, para-dichlorodiphenyldichloroethylene, 95% CI=0.546-2.551, P=0.003), and o'p'-DDD (ortho, para-dichlorodiphenyldichloroethane, 95% CI=0.004-0.690, P=0.047) were observed in maternal blood of PTB cases as compared to term delivery. The mRNA expressions of COX-2 and TNF-alpha genes were 3.13 and 2.31 folds higher in PTB cases in comparison to term delivery. Linear positive correlations were observed between period of gestation (POG) and Delta Ct of COX-2 and TNF-alpha genes.
Interpretation & conclusions: Environmental factors such as OCPs may be associated with inflammatory events showing gene-environment interaction in PTB cases. Evaluating the molecular control of inflammation along with gene environment interaction may be used as a model to explore the aetiology of idiopathic PTB cases and may be considered for the prognosis of adverse reproductive outcomes.

• 出版日期2016-6