A novel ECL biosensor for the detection of concanavalin A based on glucose functionalized NiCo2S4 nanoparticles-grown on carboxylic graphene as quenching probe

作者：Li, Xiaojian; Wang, Yaoguang; Shi, Li; Ma, Hongmin; Zhang, Yong; Du, Bin; Wu, Dan^*; Wei, Qin

来源：Biosensors and Bioelectronics, 2017, 96: 113-120.

DOI：10.1016/j.bios.2017.04.050

• Electrochemiluminescence biosensor
• Quenching
• Chitosan/Ru(bpy)(3)(2+)/silica/Fe3O4 nanomaterials
• Glucose functionalized NiCo2S4 nanoparticles grown on carboxylic graphene
• Specific carbohydrate-Con A interaction

An electrochemiluminescence (ECL) biosensor was developed for detection of Concanavalin A (Con A). Chitosan/Ru(bpy)(3)(2+)/silica/Fe3O4 nanomaterials (CRuSi-Fe3O4) were synthesized through W/O microemulsion route. The added Fe3O4 nanoparticles can simplify the prepared process and enhance the conductivity of nanomaterials which can increase the ECL intensity of luminophor CRuSi-Fe3O4. In addition, the layered structure of CRuSi-Fe3O4 can immobilize lots of Con A using glutaraldehyde as the coupling agent which can improve the sensitivity of the biosensor. Then the quenching probe glucose functionalized NiCo2S4 nanoparticles- grown on carboxylic graphene (NiCo2S4-COOH-rGO@Glu) was anchored on the modified-electrode via the specific carbohydrate-Con A interaction. Here, NiCo2S4 was used to quench the ECL of CRuSi-Fe3O4, graphene was used to grow NiCo2S4 nanoparticles as carrier materials and glucose was served as the recognition element for bounding Con A. Therefore, a desirable quenching ECL signal was measured with S2O82- as the coreactant of CRuSi-Fe3O4. Under the optimization of determination conditions, a linear response range for Con A from 0.5 pg mL(-1) to 100 ng mL(-1) was obtained, and the detection limit was calculated to be 0.18 pg mL(-1) (S/ N = 3).

• 出版日期2017-10-15
• 单位济南大学; 山东大学