Adipose-Derived Stromal Cells from Lipomas: Isolation, Characterisation and Review of the Literature

作者：Tremp Mathias; Menzi Nadia; Tchang Laurent; di Summa Pietro G; Schaefer Dirk J; Kalbermatten Daniel F^*

来源：Pathobiology, 2016, 83(5): 258-266.

DOI：10.1159/000444501

• Adipocytes
• Lipomatosis
• Proliferation
• Stem cells
• Tissue engineering

Objective: The aim of this study was to characterize adipose-derived stromal cells (ADSCs) from patients diagnosed with multiple symmetric lipomatosis (MSL) in order to obtain potentially new insights into the pathophysiology, pathogenesis and treatment of this disease. Methods: Cells from the stromal vascular fraction were analysed by the colony-forming efficiency assay and flow cytometry using standard markers. Moreover, the power of adipogenic plasticity was evaluated. Finally, a literature review was performed from 1982 to 2015 using the US National Institutes of Health's PubMed database. Results: Three European-descent patients diagnosed with either MSL type I or II could be identified for analysis. The resulting mean colony-forming efficiency assay was 14.3 +/- 5%. Flow-cytometric analysis of the ADSCs revealed high levels of CD34 (70 +/- 9%), CD45 (37 +/- 13%) and CD73 (55.8 +/- 14%), whereas low levels of CD31 (16.8 +/- 14%) and CD105 (5.8 +/- 0.7%) were detected. Furthermore, ADSCs showed a strong adipogenic potential, which is in line with the literature review. The stem cell pool in lipoma shows several alterations in biological activities, such as proliferation, apoptosis and stemness. Conclusions: ADSCs from lipoma may be interesting in the application of regenerative medicine. We discuss possible molecular treatment options to regulate their activities at the source of the MSL.

• 出版日期2016