A CO2-enriched atmosphere improves in vitro growth of Brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen]

作者：Saldanha Cleber Witt; Otoni Caio Gomide; Notini Marcela Morato; Kuki Kacilda Naomi; Ferreira da Cruz Ana Claudia; Rubio Neto Aurelio; Carnevalli Dias Leonardo Lucas; Otoni Wagner Campos^*

来源：In Vitro Cellular and Developmental Biology - Plant, 2013, 49(4): 433-444.

DOI：10.1007/s11627-013-9529-5

• Gas exchange
• Pfaffia glomerata
• Photoautotrophic growth
• Secondary metabolites
• Sucrose-free medium

The aim of the present study was to evaluate the effects of forced ventilation and CO2 enrichment (360 or 720 mu mol mol(-1) CO2) on the in vitro growth and development of Pfaffia glomerata, an endangered medicinal species, under photomixotrophic or photoautotrophic conditions. P. glomerata nodal segments showed substantial differences in growth, relative water content and water loss from leaves, photosynthetic pigments, stomatal density, and leaf anatomical characteristics under these different treatments. CO2 enrichment led to increased photosynthetic pigments and reduced stomatal density of in vitro cultivated P. glomerata. A lack of sucrose in the culture medium increased 20-hydroxyecdysone levels, but the increase in CO2 levels did not further elevate the accumulation of 20-hydroxyecdysone. All growth increased in a CO2-enriched atmosphere. In addition, CO2 enrichment, with or without sucrose, gave a lower relative water loss from leaves. This finding indicates that either a photoautotrophic or photomixotrophic system in a CO2-enriched atmosphere may be suitable for large-scale propagation of this species.

• 出版日期2013-8