Genetic diversity analysis of Capsicum spp germplasm bank accessions based on alpha/beta-esterase polymorphism

作者：Monteiro E R; Bronzato A R; Orasmo G R; Lopes A C A; Gomes R L F; Mangolin C A; Machado M F P S^*

来源：Genetics and Molecular Research, 2013, 12(2): 1155-1167.

DOI：10.4238/2013.April.12.2

• Peppers
• Capsicum spp
• Esterase
• Isozymes
• Genetic diversity

Genetic diversity and structure were analyzed in 10 accessions belonging to Banco Ativo de Germoplasma de Capsicum located at Federal University of Piaui northwestern Brazil that receives pepper samples grown in community gardens in various regions and Brazilian states. Selections were made from seeds of C. chinense (4 accessions), C. annuum (5 accessions), and C. baccatum (1 accession). Samples consisting of leaves were collected from 4-10 plants of each accession (a total of 85 plants). Native polyacrylamide gel electrophoresis was used to identify alpha-and beta-esterase polymorphisms. Polymorphism was clearly detected in 5 loci. Sixteen alleles were found at 5 alpha/beta-esterase loci of the three Capsicum species. In the C. chinense samples, the highest H-O and HE values were 0.3625 and 0.4395, respectively, whereas in C. annuum samples, H-O and HE values were 0.2980 and 0.3310, respectively; the estimated H-O and HE values in C. chinense samples were higher than those detected in C. annuum samples. A deficit of homozygous individuals was found in C. chinense (F-IS = -0.6978) and C. annuum (F-IS = 0.7750). Genetic differentiation between C. chinense and C. annuum at these loci was high (F-ST = 0.1867) indicating that C. chinense and C. annuum are genetically structured species for alpha/beta-esterase isozymes. The esterase analysis showed high genetic diversity among the C. chinense and C. annuum samples and very high genetic differentiation (F-ST = 0.6321) among the C. chinense and C. annuum samples and the C. baccatum accession.

• 出版日期2013