Histopathological features and expression profiles of cytokines, chemokines and SOCS family proteins in trehalose 6,6'-dimycolate-induced granulomatous lesions

作者：Sakai Yusuke^*; Uchida Kazuyuki; Nakayama Hiroyuki

来源：Inflammation Research, 2011, 60(4): 371-378.

DOI：10.1007/s00011-010-0280-7

• Trehalose 6,6 '-dimycolate
• Granuloma regression
• Cytokine
• Chemokine
• Suppressor of cytokine signaling family proteins
• Histopathology

The objective of this paper is to elucidate the factors contributing to the development and regression of trehalose 6,6'-dimycolate (TDM)-induced model of tuberculous granulomatous lesions.
BALB/c mice were twice injected i.p. with a 100 mu l of w/o/w emulsion (100 mu g of TDM, 3.2 mu l of Freund's incomplete adjuvant, 3.2 mu l of PBS, and 93.6 mu l of saline containing 0.2% Tween 20) at a 1 week interval. The mice were killed at days 0, 3, 7, 14, or 21 after the last injection. Three mice were used per group.
We examined histopathological changes of the lesions and defined the expression levels of cytokines and suppressor of cytokine signaling (SOCS) family proteins by real-time PCR.
The levels of inflammatory cytokine, such as TNF-alpha and IL-1 beta, paralleled with the size of the lesions and the levels of TGF-beta and SOCS-3 were high at regression phase.
Our results demonstrated that both the down-regulation of inflammatory cytokines and up-regulation of TGF-beta and SOCS-3 are crucial for histopathological changes including alteration in the sizes of the lesions and changes in inflammatory cell populations.

• 出版日期2011-4