Alteration of the Content of Primary and Secondary Metabolites in Strawberry Fruit by Colletotrichum nymphaeae Infection

作者：Mikulic Petkovsek Maja^*; Schmitzer Valentina; Slatnar Ana; Weber Nika; Veberic Robert; Stampar Franci; Munda Alenka; Koron Darinka

来源：Journal of Agricultural and Food Chemistry, 2013, 61(25): 5987-5995.

DOI：10.1021/jf402105g

• Fragaria x ananassa
• strawberry anthracnose
• fungus
• inoculation
• sugars
• organic acids
• phenolics
• resistance

The effects of infection with Colletotrichum nymphaeae, the causative agent of strawberry black spot, were studied on two strawberry cultivars: susceptible 'Elsanta' and tolerant 'Honeoye' cultivars. Four treatments were established: (1) artificial inoculation; (2) spray application of pyraclostrobin + boscalid (Signum); (3) foliar spraying with calcium (Stopit); and (4) control (spraying with water). Primary metabolites (sugars and organic acids) and secondary metabolites (phenolic compounds) were determined in strawberry fruit with the use of HPLC-MSn. Infected fruit accumulated large amounts of total sugars and low levels of organic acids. The sugar/acid ratio was higher in the infected and in Ca-treated strawberries. The contents of ellagic acid derivatives, flavonols, oligomeric procyanidins, flavan-3-ols, and total phenolics were highest in inoculated strawberry fruit. Results indicated that fungicide and calcium sprayings did not alter polyphenolic levels in plant tissue.

• 出版日期2013-6-26