MicroRNA-520a suppresses the proliferation and mitosis of HaCaT cells by inactivating protein kinase B

作者：Wang, Rui; Zhao, Zigang; Zheng, Liqiang; Xing, Xiaojing; Ba, Wei; Zhang, Junfen; Huang, Min; Zhu, Wenwei; Liu, Bing; Meng, Xianfu; Bai, Jia; Li, Chengxin; Li, Hengjin^*

来源：Experimental and Therapeutic Medicine, 2017, 14(6): 6207-6212.

DOI：10.3892/etm.2017.5323

• microRNA
• psoriasis
• proliferation
• mitosis
• keratinocytes

Psoriasis is a chronic inflammatory disease of the skin for which an effective treatment strategy remains to be developed. Characteristics of psoriasis include an altered differentiation of keratinocytes and hyperplasia of the skin. The present study aimed to investigate the role served by miR-520a in psoriasis. The results demonstrated that miR-520a inhibited the proliferation of HaCaT cells. miR-520a directly regulated the mRNA and protein expression of its target gene, protein kinase B (AKT). The siRNA silencing of AKT expression in these cells was also evaluated. miRNA-520a repressed the proliferation and mitotic entry of HaCaT cells, and promoted cell apoptosis. AKT silencing suppressed the proliferation of HaCaT cells. These results suggest that miRNA-520a regulates the survival of HaCaT cells by inhibiting AKT expression. miRNA-520a and AKT may therefore be novel targets for the treatment of patients with psoriasis.

• 出版日期2017-12
• 单位中国人民解放军总医院