A nnovel wide field fluorescence sectioning microscope based on polarization filtering image enhancement and dynamic speckle illumination

作者：Wen Qiao; Wang Kai-Ge; Shao Yong-Hong; Qu Jun-Le^*; Niu Han-Ben

来源：Acta Physica Sinica, 2013, 62(3): 034203.

DOI：10.7498/aps.62.034203

• laser speckle
• polarization filtering
• fluorescence microscope
• optical sectioning

We propose a novel wide-field fluorescence sectioning microscope based on polarization filtering image enhancement and dynamic speckle illumination in this article. A new type of polarization filtering image enhancement technology, based on the differences in polarization characteristic between excitation light and fluorescence, is used to filter out the excitation light and enhance image quality. The dynamic speckle illumination is employed to achieve the wide field fluorescence sectioning which has many advantages, such as simple configuration, low cost, rapid response and easy operation. The experimental results indicate that our filtering method can be utilized to improve the image quality significantly, and the dynamic using speckle illumination can be employed to achieve optical sectioning with a wide field and a high vertical resolution capability. Our study is not only to enrich the technological method to extract the weak fluorescence signal from the strong excitation light, but also to provide a significant reference for developing a light wavelength tunable multi-spectral fluorescence microscope with rapid response.

• 出版日期2013-2
• 单位深圳大学