A Sanger/pyrosequencing hybrid approach tor the generation of high-quality draft assemblies of marine microbial genomes

作者：Goldberg Susanne M D^*; Johnson Justin; Busam Dana; Feldblyum Tamara; Ferriera Steve; Friedman Robert; Halpern Aaron; Khouri Hoda; Kravitz Saul A; Lauro Federico M; Li Kelvin; Rogers Yu Hui; Strau**erg Robert; Sutton Granger; Tallon Luke; Thomas Torsten; Venter Eli; Frazier Marvin; Venter J Craig

来源：Proceedings of the National Academy of Sciences of the United States of America, 2006, 103(30): 11240-11245.

DOI：10.1073/pnas.0604351103

Since its introduction a decade ago, whole-genome shotgun sequencing (WGS) has been the main approach for producing cost-effective and high-quality genome sequence data. Until now, the Sanger sequencing technology that has served as a platform for WGS has not been truly challenged by emerging technologies. The recent introduction of the pyrosequencing-based 454 sequencing platform (454 Life Sciences, Branford, CT) offers a very promising sequencing technology alternative for incorporation in WGS. In this study, we evaluated the utility and cost-effectiveness of a hybrid sequencing approach using 3730xl Sanger data and 454 data to generate higher-quality lower-cost assemblies of microbial genomes compared to current Sanger sequencing strategies alone.

• 出版日期2006-7-25