A cDNA encoding a chitinase from the epithelial cell line of Chironomus tentans (Insecta, Diptera) and its functional expression

作者：Feix M; Gloggler S; Londershausen M; Weidemann W; Spindler KD; Spindler Barth M^*

来源：Archives of Insect Biochemistry and Physiology, 2000, 45(1): 24-36.

DOI：10.1002/1520-6327(200009)45:1<24::AID-ARCH3>3.3.CO;2-8

• insect
• enzyme
• Baculovirus/Spodoptera

A cDNA coding for chitinase was isolated from Chironomus cells, which possesses conserved regions I and II characteristic for family 18 chitinases, a C-terminus enriched in Glu and Pro without the typical "PEST-region," putative glycosylation sites, a reduced number of C-terminal cysteines, and no typical chitin binding domain. Northern blots revealed one specific signal with an apparent size of 2.3 kb. The cDNA was expressed in the baculovirus/Spodoptera system as a His-tag fusion protein, which was secreted as a functionally active enzyme into the medium and could be separated from endogenous viral and Spodoptera-specific chitinases. Arch. Insect Biochem, Physiol. 45:24-36, 2000.

• 出版日期2000-9