A proteomic analysis of transplanted liver in a rat model of chronic rejection

作者：Wei, Wei; Huang, Xin-Hui; Liang, Dong; Zeng, Yong-Yi; Ma, Chuang; Wu, Yan-Bin; Li, Yun-Tong; Zhang, Xiang; Zeng, Jin-Hua; Liu, Jing-Feng^*

来源：Clinics and Research in Hepatology and Gastroenterology, 2015, 39(3): 340-350.

DOI：10.1016/j.clinre.2014.10.005

• GELATINASE-ASSOCIATED LIPOCALIN
• AMINO-ACID-METABOLISM
• EXPRESSION
• CLUSTERIN
• BIOMARKERS
• PROTEINS
• IMMUNOSUPPRESSION
• IDENTIFICATION
• QUANTITATION
• TACROLIMUS

Background: Chronic rejection (CR) is an important cause of liver allograft failure. In the latter condition, re-transplantation of the liver (ReLT) is the only option for survival. Unfortunately, with the current state of knowledge, it is difficult to diagnose and treat early CR.
Objective: To explore the biomarkers of the chronic rejection in orthotopic liver transplantation (OLT).
Methods: A rat model of chronic liver allograft rejection was established, and the differential protein expression in chronic allograft rejection (CR) was analyzed by iTRAQ-MALDI-TOF/TOF.
Results: Expression of sixty-two proteins was found to be significantly changed in CR rats. In the present study, CLU, Lcn2 and Krt19 were identified and quantified as early and reliable biomarkers for chronic rejection.
Conclusion: Analysis of differential protein expression by iTRAQ-MALDI-TOF/TOF is a potentially effective method to help understand the mechanism of CR in orthotopic liver transplantation. The proteins CLU, Lcn2 and Krt19 might be potential prognostic markers for predicting chronic rejection after liver transplantation.

• 出版日期2015-6
• 单位福建医科大学