科研之友
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摘要
The metabolic properties of carbon sources and potential functional diversity of the microbial community in horizontal subsurface flow-constructed wetlands (HSSF) was analyzed through the sole-carbon-source utilization profiles using BIOLOG-ECO microplates This constructed wetland (CW) was treating rural eutrophic water in China The plants used in the CW were Typha angustifolia This study showed that the metabolic activities of upper and front substrate microorganisms were greater than those of rear substrate microorganisms in the constructed wetland The substrate microorganisms in the front and upper part of CW showed 58 9%, 11 8%, and 41 4% higher growth rates of integrated areas under average well color development (AWCD) curves than in the front and lower part, the rear and upper part, and the rear and lower part of the CW, respectively By principal component analysis (PCA), it was shown that there existed significant differences in community structure and metabolic properties of carbon sources between substrate microorganisms from different sampling sites Carbon source utilization of polymers, carbohydrates, carboxylic acids and amino acids were greater in all sampling sites, but that of amines and phenolic compounds were very different The richness, evenness and diversity of upper substrates in microbial communities were significantly higher than those of lower substrate The lower front substrate microbial communities had lower evenness than the other sampling plots, and the lowest richness of substrate in microbial community was found in the lower back-end part of the constructed wetlands
