A new link between steroid resistance, glucocorticoid receptor and nuclear factor kappa B p65 in idiopathic nephrotic syndrome

作者：Szilagyi Katarina^*; Podracka Ludmila; Franke Niels E; Mojzis Jan; Mirossay Ladislav

来源：Neuroendocrinology Letters, 2009, 30(5): 629-636.

• glucocorticoids
• sensitivity
• relapse
• peripheral blood cells
• idiopathic nephrotic syndrome

OBJECTIVES: The aim of our study was to investigate levels of glucocorticoid receptor (GR alpha), nuclear factor kappa B (NF kappa B) p65/p50 and inhibitor of NF kappa B alpha (I kappa B alpha) in the peripheral mononuclear blood cells (PMBC) of children with idiopathic nephrotic syndrome (INS).
METHODS: The expression of GR alpha, NFKBp65/p50 and I kappa B alpha was determined in 59 patients (age 10.2 +/- 5.1) and 25 healthy controls (CO) (age 13.1 +/- 3.4) using Western blot analysis. Patients were labeled according to their clinical sensitivity to glucocorticoids (GCs) as responders (RE), partial responders (PR), and non-responders (NR).
RESULTS: Significantly higher expressions of GR alpha were observed in RE than in PR, NR (p<0.01) and even CO (p<0.05). Similarly, expression of NF kappa Bp65 was higher in RE in comparison to PR, NR and CO (p<0.05). These differences were more emphasized in the relapse: levels of GR alpha were significantly lower in PR than in RE and CO (p<0.01). Significant differences were also observed in expression of NF kappa B: RE showed significantly higher expression of NF kappa Bp65 in comparison to PR, NR and even CO (p<0.01).
CONCLUSIONS: Lower levels of both GR alpha and NF kappa Bp65 are associated with poor or no response to GCs and the difference is more pronounced in patients experiencing relapse of INS.

• 出版日期2009